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عمومی::
دنباله جرملاین انسانی
As is understood in the art, the CDRs are separated by framework regions in each of the variable heavy and variable light domains: for the light variable domain, these are FR1-vlCDR1-FR2-vlCDR2-FR3-vlCDR3-FR4, and for the heavy variable domain, these are FR1-vhCDR1-FR2-vhCDR2-FR3-vhCDR3-FR4, with the framework regions showing high identity to human germline sequences.
Typically, a humanized antibody derived from a particular human germline sequence will display no more than 10-20 amino acid differences from the amino acid sequence encoded by the human germline immunoglobulin gene.
In this embodiment, preferred variants in the framework regions (e.g. excluding the CDRs) retain at least about 80, 85, 90 or 95% identity to a human germline sequence.
Thus, for example, the identical CDRs as described herein can be combined with different framework sequences from human germline sequences, as long as the framework regions retain at least 80, 85, 90 or 95% identity to a human germline sequence.
, as well as having framework region changes, as long as the framework regions retain at least 80, 85 or 90% identity to a human germline sequence, and the resulting CDRs are not to the murine CDRs outlined in PCT/JP13/65090 and shown in SEQ-ID-NOs:81, 82 and 83 (variable light CDRs) and SEQ-ID-NOs:86, 87 and 88 (variable heavy CDRs).
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